PI3K p85 alpha/gamma ELISA Kit

Catalog No. ABIN8085144

The Human, Mouse and Rat PI3K p85 alpha/gamma ELISA Kit (ABIN8085144) is a Colorimetric ELISA Kit designed to quantify Human, Mouse and Rat PI3K p85 alpha/gamma.

Quick Overview for PI3K p85 alpha/gamma ELISA Kit (ABIN8085144)

Target: PI3K p85 alpha/gamma (PI3K p85a/g) (Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g))

Binding Specificity: pTyr199, pTyr467

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details PI3K p85 alpha/gamma ELISA Kit

Purpose: Phospho-PI3-kinase p85-α/γ (Tyr467/199) Colorimetric Cell-Based ELISA Kit for detecting Phospho-PI3-kinase p85-α/γ (Tyr467/199) in researchers plate their cell line of choice.

Analytical Method: Qualitative

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 4 - 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 6 months

Target Details for PI3K p85 alpha/gamma

Target: PI3K p85 alpha/gamma (PI3K p85a/g) (Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g))

Alternative Name: PI3-kinase p85-alpha/gamma

Background: Gene Aliases: GRB1, P85A, PI3-kinase p85-alpha subunit, PI3K, PI3K p85-alpha, Phosphatidylinositol 3-kinase regulatory alpha subunit, PtdIns-3-kinase p85-alpha, P55G, PI3-kinase p85-gamma subunit, PI3K p85-gamma, Phosphatidylinositol 3-kinase regulatory gamma subunit, PtdIns-3-kinase p85-gamma, p55PIK

Gene ID: 5295

UniProt: P27986