WFDC2 ELISA Kit

Catalog No. ABIN5692224

Rat WFDC2 ELISA Kit, Colorimetric assay for quantification of Rat WFDC2.

Quick Overview for WFDC2 ELISA Kit (ABIN5692224)

Target: WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))

Binding Specificity: AA 31-168

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details WFDC2 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of rat HE4. 96wells/kit, with removable strips.

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli, Immunogen sequence: E31-F168

Sensitivity: < 10 pg/mL

Components: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Application Details

Assay Time: 15 min

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Expiry Date: 12 months

Target Details for WFDC2

Target: WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))

Alternative Name: Wfdc2

Background:

Synonyms: WAP four-disulfide core domain protein 2, Epididymal secretory protein 4, RE4, Wfdc2

Background: WAP four-disulfide core domain protein 2, also known as Human Epididymis Protein 4 (HE4), is a protein that in humans is encoded by the WFDC2 gene. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.

Cellular Localisation: Secreted.

UniProt: Q8CHN3