IL12A ELISA Kit

Catalog No. ABIN5692238

Product Details IL12A ELISA Kit

Target: IL12A (Interleukin 12 alpha (IL12A))

Binding Specificity: AA 23-215, AA 23-335

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-12(P70). 96wells/kit, with removable strips.

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: Expression system for standard: sf21, Immunogen sequence: (M23-S335)+(R23-S215)

Sensitivity: < 1.5 pg/mL

Components: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Application Details

Assay Time: 15 min

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Expiry Date: 12 months

Target Details for IL12A

Target: IL12A (Interleukin 12 alpha (IL12A))

Alternative Name: Il12a (IL12A Products)

Synonyms: CLMF ELISA Kit, IL-12A ELISA Kit, NFSK ELISA Kit, NKSF1 ELISA Kit, P35 ELISA Kit, CLMF2 ELISA Kit, IL-12B ELISA Kit, NKSF ELISA Kit, NKSF2 ELISA Kit, p40 ELISA Kit, Il-12b ELISA Kit, Il12p40 ELISA Kit, Il-12p40 ELISA Kit, Il12 ELISA Kit, IL-12p40 ELISA Kit, IL-12p35 ELISA Kit, Il-12a ELISA Kit, Ll12a ELISA Kit, p35 ELISA Kit, IL12p35 ELISA Kit, Il-12 p35 ELISA Kit, IL12p40 ELISA Kit, IL12 ELISA Kit, IL-12 p35 ELISA Kit, interleukin 12A ELISA Kit, interleukin 12B ELISA Kit, interleukin 12b ELISA Kit, interleukin 12a ELISA Kit, Interleukin 12 level ELISA Kit, IL12A ELISA Kit, IL12B ELISA Kit, Il12b ELISA Kit, Il12a ELISA Kit, IL12 ELISA Kit, il12a ELISA Kit

Background:

Synonyms: Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, CLMF p35, IL-12 subunit p35, Il12a

Background: Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits, it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant rat IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.

Cellular Localisation: Secreted.

UniProt: Q9R103

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation