IL2 Receptor beta ELISA Kit

Catalog No. ABIN5706310

Mouse IL2 Receptor beta ELISA Kit, Colorimetric assay for quantification of Mouse IL2 Receptor beta.

Quick Overview for IL2 Receptor beta ELISA Kit (ABIN5706310)

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Product Details IL2 Receptor beta ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Mouse IL-2sRβ/CD122 (Soluble Interleukin-2 Receptor Beta Chain) ELISA Kit

Analytical Method: Quantitative

Specificity: Specifically binds with IL-2sRβ/CD122 , no obvious cross reaction with other analogues.

Sensitivity: 37.5 pg/mL

Components:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antibody
• HRP-Streptavidin Conjugate(SABC
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Material not included:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Assay Time: 4 h

Plate: Pre-coated

Protocol: Step 1: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate twice without immersing. Step 2: Add 100 µL biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid direct light exposure to antibody solutions

Storage: 4 °C

Storage Comment: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Expiry Date: 12 months

Target Details for IL2 Receptor beta

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Alternative Name: Soluble Interleukin-2 Receptor Beta Chain

Background: SIL-2RB, Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL-2R subunit beta, , IL-2RB, High affinity IL-2 receptor subunit beta, p70-75, CD122, Il2rb

UniProt: P16297

Pathways: JAK-STAT Signaling, Growth Factor Binding