H+K+-ATPase Activity Assay Kit

Catalog No. ABIN7121129

Quick Overview for H+K+-ATPase Activity Assay Kit (ABIN7121129)

Target: ATP4b (ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b))

Detection Method: Colorimetric

Application: Activity Assay (AcA)

Sample Type: Cell Samples, Tissue Samples

Analytical Method: Quantitative

Product Details

Purpose: The H+/K+-ATPase Assay Kit is biochemical kit in Colorimetric method

Characteristics: ATPase can decompose ATP to produce ADP and inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. The inorganic phosphorus reacts with ammonium molybdate in acidic solution to form ammonium molybdate compound, which is reduced with reducing agent to form molybdenum blue, and has absorption peak at 660 nm. Determine the concentration of molybdenum blue to calculate the amount of inorganic phosphorus.

Material not included: Spectrophotometry (Visible range)

Application Details

Assay Time: 1 h

Assay Precision:

Inter-assay precision: 9.8%

Intra-assay precision: 4.4%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: 4 °C,-20 °C

Storage Comment: This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

Expiry Date: 12 months

Target Details

Target: ATP4b (ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b))

Alternative Name: H+K+-ATPase