Interferon gamma ELISA Kit

Catalog No. ABIN577086

The Human Interferon gamma ELISA Kit (ABIN577086) is a Colorimetric ELISA Kit designed to quantify Human Interferon gamma.

Quick Overview for Interferon gamma ELISA Kit (ABIN577086)

Target: Interferon gamma (IFNG)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.5-1600 pg/mL

Application: ELISA

Product Details Interferon gamma ELISA Kit

Minimum Detection Limit: 7.5 pg/mL

Purpose: This IFN- enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal specific for IFN- . Standards or samples are then added - the appropriate microtiter plate wells and incubated. IFN- if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed - remove unbound IFN- and other components of sample. In order - quantitatively determine the amount of IFN- present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody specific for IFN- is added - each well to

Analytical Method: Quantitative

Sensitivity: < 7.5 pg/mL

Components: Standards: 1 set/2 vials

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Preservative: Without preservative

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: Interferon-Gamma (IFN-y)

Background: Tumour Necrosis Factor alpha (TNF-alpha), also known as cachectin was initially named for its remarkable ability to cause hemorrhagic necrosis of tumours in mice.1 The TNF- gene located on chromosome 6 encodes a 233 amino acid (a.a.) prohormone bound to the plasma membrane, with the mature form (157 a.a., 17 kDa) exposed on the extracellular surface. Soluble, mature TNF- is released upon cleavage of the C- terminal.2,3 The primary source in vivo of TNF- is thought to be the monocyte/macrophage but various cell types are known to express this cytokine such as lymphocytes, basophils, eosinophils, mast cells, NK cells, T cells, B cells, kerantinocytes, Kupffer cells, astrocytes, and some types of tumours. TNF- is produced upon stimulation with cytokines such as IL-1, 1L-2, GM-CSF, TNF- itself and with bacterial lipopolysaccharide (LPS) which is a potent inducer.4 TNF- once produced and secreted will bind to TNF- receptors (TNF- R1, 55 kDa and TNF- R2, 75 kDa), located on the plasma membrane of most cells throughout the body except the red blood cell.5 The two TNF- receptors deliberate the biological effects of TNF- . It has been reported that TNF- R1 is responsible for mediating LPS toxicity6 and cell cytotoxicity7 and TNF- R2 is involved in cellular proliferation.8 Various pathological conditions are associated with the production of high levels of TNF- . These include septic shock syndrome, cachexia (e.g. HIV, tuberculosis, cancer), autoimmune diseases, hepatitis, leukemia, myocardial ischaemia, organ transplantation rejection, multiple sclerosis, rheumatoid arthritis, and meningococcal septicemia.4,9,1,11 Many people die annually from a complication of infectious disease called septic shock syndrome which is triggered by TNF- . In many cases elevated TNF- serum levels predict a higher mortality.12 TNF- is a pleiotropic cytokine that can induce disease through TNF- toxicity (tissue injury, catabolic illness, and mediating shock) and improve host defense mechanisms (stimulating inflammation and increasing immune cell function).4 In the future, therapies may be developed by blocking TNF- harmful effects and enhancing TNF- beneficial effects. This TNF- ELISA is a 2.5-3 hour solid phase immunoassay readily applicable to measure TNF- levels in serum, plasma, cell culture supernatant, and other biological fluids in the range of to 1 pg/mL. It showed no cross reactivity with other cytokines tested. This TNF- ELISA is expected to be effectively used for further investigations into the relationship between TNF- and the various conditions mentioned. S7.5(5) TNF-

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy