CD25 ELISA Kit

Catalog No. ABIN577095

The Human CD25 ELISA Kit (ABIN577095) is a Colorimetric ELISA Kit designed to quantify Human CD25.

Quick Overview for CD25 ELISA Kit (ABIN577095)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Product Details CD25 ELISA Kit

Purpose: This IL-2 sR enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-2 sR . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-2 sR and incubated. IL-2 sR if present, will bind and become immobilized by the antibody pre-coated on the wells and then become

Analytical Method: Quantitative

Sensitivity: The minimum detectable quantity of human IL-2 sR as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II is 10 pg/mL. The two standard deviations above the mean optical density of the 20 replicates of the zero standard were defined as the minimum detectable quantities.

Components: Standards: 1 set/2 vials

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Preservative: Without preservative

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: Interleukin-2 Soluble Receptor alpha (IL-2 sRa)

Background: The mature human IL-1 is a protein with 16 amino acids and the functional IL-1 is a homodimer. IL-1 is expressed by a variety of cells, including mouse T cells (TH1, TH2, and Tr1 subsets), B cells derived from peripheral blood, tonsils or spleen, Epstein Barr virus (EBV)-transformed B cell lines, Burkitt's lymphoma, AIDS lymphomas, monocytes, placental trophoblasts, bronchial epithelial cells, and certain tumor cells including melanomas and carcinomas of various origin. IL-1 plays an important role in inflammatory and immune responses. The biological activities of IL-1 include both immunosuppressive and immuno-stimulatory effects. For example, IL-1 can suppress the production of pro-inflammatory cytokines by monocytes and neutrophils, and down regulates the expression of activating and co-stimulatory molecules on monocytes and dendritic cells. IL-1 also can improve the growth of B cells and mast cells, and inhibit or enhance the activities of T cells depending on their activation conditions. The different polymorphisms in the IL-1 gene promoter have been demonstrated the association with both SIDS (sudden infant death syndrome) and sudden unexpected death due to infection. Based on the functions of IL-1, it has been suggested that an IL-1 antagonist can be applied to boost anti-viral immunity against viruses such as EBV and that the IL-1 molecule itself may be used as an anti-inflammatory reagent. This IL-1 ELISA is a ready-to-use 4.5-hour solid phase immunoassay capable of measuring IL-1 levels in serum, plasma, cell culture supernatant, and other biological fluids in the range of to 16 pg/mL. This assay has shown no cross-reactivity with other cytokines tested, and is expected to be used effectively for further investigations into the relationship between IL-1 and the various conditions mentioned

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation