IL-11 ELISA Kit

Catalog No. ABIN577100

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL-11.

Quick Overview for IL-11 ELISA Kit (ABIN577100)

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Product Details IL-11 ELISA Kit

Purpose: This IL-11 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-11. Standards or samples are then added to the appropriate microtiter plate wells and incubated. After washing to remove unbound IL-11 and other components of the sample, biotin-conjugated polyclonal antibody specific to IL-11 is added and incubated. If present, IL-11 will bind and become immobilized by the antibody pre-coated on the wells and then become

Analytical Method: Quantitative

Sensitivity: The minimum detectable quantities of human IL-10 as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II are 4.0 pg/mL and 3.0pg/mL respectively. The two standard deviations above the mean optical density of the 16 replicates of the zero standard were defined as the minimum detectable quantities.

Components: Standards: 1 set/2 vials

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Preservative: Without preservative

Target Details for IL-11

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Alternative Name: Interleukin-11 (IL-11)

Background: Human chorionic gonadotropin is a glycoprotein hormone produced by normal trophoblast cells of the placenta during pregnancy. It is also is produced by trophoblast cells in hydatidiform mole and choriocarcinoma (trophoblast diseases), and in patients with germ cell tumors (testicular choriocarcinoma, placental site tumors and germ cell carcinomas of the ovary) and sometimes in those with other malignancies. Small amount of hCG may also be produced by the pituitary gland. Human CG (hCG) is the hormone associated with the maintenance of pregnancy. Human chorionic gonadotropin is a member of the glycoprotein hormone (GPH) family. Like the other glycoprotein hormones, follicle stimulating hormone (FSH), luteinizing hormone (LH) and thyroid stimulating hormone (TSH), hCG is composed of two subunits, alpha and beta. Alpha subunits of these various glycoprotein hormones are structurally very similar, but beta subunits differ in amino acid sequences. These differences are responsible for their biological and immunological specificity. The alpha- subunit of hCG is a glycopeptide of 92 amino acids and the -subunit of hCG is a glycopeptide of 145 amino acids. HCG has a molecular weight of 5, daltons, consists of an alpha subunit of 18, daltons and a beta subunit of 32, daltons. Recent elucidation of the crystal structure of hCG has revealed that all these subunits share the so-called cystin-knot structural motif with growth factors such as nerve (NGF), platelet-derived (PDGF). Although the pituitary secretes three related glycoprotein hormones, LH, FSH, and TSH, hCG is the only one to produced by the placenta in primates to maintain the steroid hormone secretions of the corpus luteum. Predominantly intact hCG is present in serum and urine samples in normal pregnancy, as well as in hydatidiform mole. In women with extrauterine or ectopic pregnancies, unduly low hCG levels may be detected. Variable levels of hCG mostly intact hCG, may be present from individuals with persistent trophoblastic disease. Elevated concentrations are also present in cases of early pregnancy loss (EPL) or biochemical pregnancy, gestational Down syndrome. Bladder cancer, ovarian cancer and certain other malignancies may generate a small amount of hCG alpha and beta subunit. Commonly, the amount of subunit is insufficient for combination to occur to make intact hCG. S7.5(2) hCG _x000C_ PRINCIPLES OF THE ASSAY This hCG enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre- coated with a monoclonal antibody specific to hCG. Standards or samples are added to the microtiter plate wells and incubated. The plate wells are washed to remove any unbound standard or sample, hCG, if present, will bind to the antibody pre-coated on the wells. A standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody specific to hCG is added to each well to

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion