M-CSF/CSF1 ELISA Kit

Catalog No. ABIN577102

Human M-CSF/CSF1 ELISA Kit, Colorimetric assay for quantification of Human M-CSF/CSF1.

Quick Overview for M-CSF/CSF1 ELISA Kit (ABIN577102)

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Product Details M-CSF/CSF1 ELISA Kit

Purpose: This M-CSF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to M-CSF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for M-CSF and incubated. If present, M-CSF will bind and become immobilized by the antibody pre-coated on the wells and then become

Analytical Method: Quantitative

Sensitivity: The minimum detectable quantities of human M-CSF as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II are 15 pg/mL and 37pg/mL respectively. The two standard deviations above the mean optical density of the 20 replicates of the zero standard were defined as the minimum detectable quantities. S7.5(02) M-CSF 11

Components: Standards: 1 set/2 vials

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Preservative: Without preservative

Target Details for M-CSF/CSF1

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Alternative Name: Macrophage Colony Stimulating Factor (M-CSF)

Background: The biological function of IL-2 is obtained by binding to the specific interleukin-2 receptor (IL-2R). The IL-2R consists of three non-covalently linked chains, all of which are type I transmembrane proteins and include the chain (IL-2R , p55), chain (IL-2R , p75), and chain (IL-2R , p65). The chain is cleaved from the cell surface via nonspecific proteolysis. IL-2R and IL-2R dimers bind to different residues on the IL-2 protein. The IL-2R complex displays low affinity and the IL-2R complex displays intermediate affinity for IL- 2 binding. Both IL-2R and IL-2R complexes are unable to transduce a signal. The IL- 2R complex has intermediate affinity for IL-2 binding and can transduce a signal with a relatively high concentration of IL-2. The IL-2R trimer is the high-affinity receptor for IL- 2 and can transduce a signal successfully. Many cells are capable of expressing IL-2R including the antigen-activated T cells and B cells, and approximately 1% of natural killer (NK) cells, leukemia and lymphoma cells. When produced by activated T cells, the chain is? 1-2 folds in excess of the and chain. A soluble IL-2R can be detected in tissue culture media of IL-2R+ cells and in the serum of experimental animals and humans undergoing an immune response. The major biological activities of IL-2R include promoting the proliferative expansion of T cells and NK cells upon activation, promoting the persistence of antigen-selected memory T cells, and promoting homeostasis of the immune system after it has successfully responded to an antigen. However, the biological activity of soluble IL-2R is unclear. It has been reported that elevated IL-2 sR level is accompanied by increased T and B cell activation and immune system activation as observed in rheumatoid arthritis, systemic lupus erythematosis (SLE), some leukemias and lymphomas. Because of its low affinity, IL-2 sR would be expected to be an inhibitor of IL-2. This IL-2 sR ELISA is a ready-to-use 4.5-hour solid phase immunoassay capable of measuring IL-2 sR levels in serum, plasma, cell culture supernatant, and other biological fluids in the range of to 2 pg/mL. This assay has shown no cross-reactivity with other cytokines tested, and is expected to be used effectively for further investigations into the relationship between IL-2 sR and the various conditions mentioned. S7.5(3) IL-2 sR 2

Pathways: RTK Signaling