PON1 ELISA Kit

Catalog No. ABIN6719699

Product Details PON1 ELISA Kit

Target: PON1 (Paraoxonase 1 (PON1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Lysate, Cell Culture Supernatant, Plasma (heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Characteristics:

Synonyms: A esterase 1, A-esterase 1, Aromatic esterase 1, Arylesterase 1, Arylesterase B type, ESA, Esterase A, K 45, K-45, MVCD5, Paraoxonase 1, Paraoxonase, Paraoxonase B type, Paraoxonase, plasma, Paraoxonase1, PON 1, PON, PON1, PON1_HUMAN, Serum aryldiakylphosphatase, Serum aryldialkylphosphatase 1, Serum paraoxonase/arylesterase 1

Background: Serum paraoxonase/arylesterase 1(PON1) also known as aromatic esterase 1 or serum aryldialkylphosphatase 1 is an enzyme that in humans is encoded by the PON1 gene. The PON gene was mapped to chromosome 7q21-q22, and the mouse Pon1 gene was mapped to the proximal end of chromosome 6. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1(paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein(HDL).

Gene Name: PON1

Production: Natural and recombinant human PON1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A2-L355

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human PON1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum, plasma(heparin) or tissue homogenates to each empty well. It is recommended that each human PON1 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for PON1

Target: PON1 (Paraoxonase 1 (PON1))

Alternative Name: PON1 (PON1 Products)

Synonyms: esa ELISA Kit, pon ELISA Kit, pon2 ELISA Kit, MGC53915 ELISA Kit, MGC89222 ELISA Kit, PON1 ELISA Kit, ESA ELISA Kit, MVCD5 ELISA Kit, PON ELISA Kit, Pon ELISA Kit, paraoxonase 2 ELISA Kit, paraoxonase 1 ELISA Kit, pon2 ELISA Kit, PON1 ELISA Kit, Pon1 ELISA Kit

Gene ID: 5444

NCBI Accession: NP_000437

UniProt: P27169