LIFR ELISA Kit

Catalog No. ABIN6719764

Product Details LIFR ELISA Kit

Target: LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: CD118, CD118 antigen, FLJ98106, FLJ99923, Leukemia inhibitory factor receptor alpha, Leukemia inhibitory factor receptor, LIF R, LIF receptor, LIF-R, Lifr, LIFR_HUMAN, SJS2, STWS, SWS

Background: LIFR, also known as CD118(Cluster of Differentiation 118), is a subunit of a receptor for leukemia inhibitory factor. This gene encodes a protein that belongs to the type I cytokine receptor family. It is mapped to 5p31.1. The LIF receptor(LIFR) is the low-affinity binding chain that, together with the high-affinity converter subunit gp130, forms a high-affinity receptor complex that mediates the action of the leukemia-inhibitory factor. LIF is a polyfunctional cytokine that affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2, a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene, togerther with the pleiomorphic adenoma gene 1, is associated with salivary gland pleiomorphic adenoma, a common type of benign epithelial tumor of the salivary gland.

Gene Name: LIFR

Production: Natural and recombinant human LIFR. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q45-S833

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human LIFR standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human LIFR standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for LIFR

Target: LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))

Alternative Name: LIFR (LIFR Products)

Synonyms: A230075M04Rik ELISA Kit, AW061234 ELISA Kit, LIF ELISA Kit, CD118 ELISA Kit, LIF-R ELISA Kit, SJS2 ELISA Kit, STWS ELISA Kit, SWS ELISA Kit, leukemia inhibitory factor receptor ELISA Kit, LIF receptor alpha ELISA Kit, leukemia inhibitory factor receptor alpha ELISA Kit, Lifr ELISA Kit, LOC397451 ELISA Kit, LIFR ELISA Kit

Gene ID: 3977

NCBI Accession: NP_001121143

UniProt: P42702

Pathways: JAK-STAT Signaling, Growth Factor Binding