VEGFB ELISA Kit

Catalog No. ABIN6719783

Supplier Product No.: koa0852

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse VEGFB.

Quick Overview for VEGFB ELISA Kit (ABIN6719783)

Target: VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (heparin), Serum

Product Details VEGFB ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0852

Supplier: Rockland

Purpose: Mouse VEGF-B Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse VEGF-B. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 31.2pg/mL-2000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse VEGF-B standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. We recommend that each mouse VEGF-B standard solution and each sample is measured in duplicate.

Comment:

Standard: Expression system for standard: sf21, Immunogen sequence: Q20-A207&Q20-R148

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for VEGFB

Target: VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

Alternative Name: VEGF-B

Background:

Synonyms: Vascular endothelial growth factor B, VEGF B, VEGF related factor, VEGF-B, VEGF-related factor, Vegfb, VEGFB_HUMAN, VEGFL, VRF

Background: Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. And it is important for the protection of neurons in the retina and the cerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.

Gene ID: 22340

NCBI Accession: NP_001172093

UniProt: P49766

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals