VEGFB ELISA Kit

Catalog No. ABIN2859260

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse VEGFB. There is 1 publication available.

Quick Overview for VEGFB ELISA Kit (ABIN2859260)

Target: VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

Binding Specificity: AA 20-148, AA 20-207

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin)

Product Details VEGFB ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich Mouse VEGF-B ELISA Kit to quantitate Mouse Vegfb in cell culture supernatants, serum and plasma (heparin). .

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: Q20-A207 & Q20-R148

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 5.0%, 7.5%, 5.0%
Inter-Assay Precision (CV%): 7.0%, 8.2%, 5.7%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for VEGFB ELISA Kit (ABIN2859260)

Zhao, Shi, Liang, Miao, Xie, Zhang, Li: "Expression of pro- and anti-angiogenic isoforms of VEGF in the mouse model of oxygen-induced retinopathy." in: Experimental eye research, Vol. 93, Issue 6, pp. 921-6, (2011) (PubMed).

Target Details for VEGFB

Target: VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

Alternative Name: VEGFB

Background:

Background: Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. And it is important for the protection of neurons in the retina and thecerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.

Gene Full Name: vascular endothelial growth factor B

Gene ID: 22340

UniProt: P49766

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals