EPO ELISA Kit

Catalog No. ABIN6719829

Product Details EPO ELISA Kit

Target: EPO (Erythropoietin (EPO))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 46.9 pg/mL - 3000 pg/mL

Minimum Detection Limit: 46.9 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Characteristics:

Synonyms: EP, EPO, EPO_HUMAN, Epoetin, Erythropoietin, MVCD2

Background: Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. This gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What's more, EPO is also involved in the wound healing process.

Gene Name: EPO

Production: Natural and recombinant rat EPO. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: T44-K226

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL,419.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL rat EPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants, serum, plasma(heparin) or tissue homogenates to each empty well. We recommend that each rat EPO standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 46.9pg/mL-3000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for EPO

Target: EPO (Erythropoietin (EPO))

Alternative Name: EPO (EPO Products)

Synonyms: EPO ELISA Kit, EP ELISA Kit, MVCD2 ELISA Kit, erythropoietin ELISA Kit, erythropoietin S homeolog ELISA Kit, erythropoietin a ELISA Kit, EPO ELISA Kit, epo ELISA Kit, epo.S ELISA Kit, Epo ELISA Kit, epoa ELISA Kit

Target Type: Hormone

Gene ID: 24335

NCBI Accession: NP_058697

UniProt: P29676

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity