Neurotrophin 3 ELISA Kit

Catalog No. ABIN6719839

Supplier Product No.: koa0280

Mouse Neurotrophin 3 ELISA Kit, Colorimetric assay for quantification of Mouse Neurotrophin 3.

Quick Overview for Neurotrophin 3 ELISA Kit (ABIN6719839)

Target: Neurotrophin 3 (NTF3)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details Neurotrophin 3 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Supplier Product No.: koa0280

Supplier: Rockland

Purpose: Mouse Neurotrophin-3 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: Y139-T257

Cross-Reactivity (Details): There is cross-reactivity with NT-4<2.5 % , and no detectable cross-reactivity with any other cytokine.

Sensitivity: < 2 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse Neurotrophin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants or serum to each empty well. It is recommended that each mouse Neurotrophin-3 standard solution and each sample be measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 7.4%, 4%, 5.6%
Inter-Assay Precision (CV%): 9.2%, 4.5%, 6%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Neurotrophin 3

Target: Neurotrophin 3 (NTF3)

Alternative Name: Neurotrophin-3

Background:

Background: Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system.NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3, also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.

Gene Full Name: neurotrophin 3

Gene ID: 18205

NCBI Accession: NP_001157506

UniProt: P20181

Pathways: RTK Signaling, Neurotrophin Signaling Pathway