ADAMTS1 ELISA Kit

Catalog No. ABIN6719945

Supplier Product No.: koa0805

This Colorimetric ELISA kit is designed for the quantitative measurement of Human ADAMTS1.

Quick Overview for ADAMTS1 ELISA Kit (ABIN6719945)

Target: ADAMTS1 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 93.8 pg/mL - 6000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (heparin), Serum

Product Details ADAMTS1 ELISA Kit

Minimum Detection Limit: 93.8 pg/mL

Supplier Product No.: koa0805

Supplier: Rockland

Purpose: Human ADAMTS1 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human ADAMTS1. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.7pg/m human ADAMTS1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human ADAMTS1 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: F253-A734

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for ADAMTS1

Target: ADAMTS1 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1))

Alternative Name: ADAMTS1

Background:

Synonyms: A disintegrin and metalloproteinase with thrombospondin motifs 1, A disintegrin like and metalloprotease with thrombospondin type 1 motif 1, A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 1, ADAM metallopeptidase with thrombospondin type 1 motif 1, ADAM TS 1, ADAM TS1, ADAM-TS 1, ADAM-TS1, ADAMTS 1, ADAMTS, ADAMTS-1, Adamts1, ATS1_HUMAN, C3 C5, KIAA1346, METH 1, METH-1, METH1

Background: ADAMTS1 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. ADAMTS1 gene is mapped to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. It has been found that ADAMTS1 can disrupt angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin. What's more, ADAMTS1 disrupts angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin.

Gene ID: 9510

NCBI Accession: NP_008919

UniProt: Q9UHI8

Pathways: SARS-CoV-2 Protein Interactome