IL15RA ELISA Kit

Catalog No. ABIN6720034

Supplier Product No.: koa0654

Mouse IL15RA ELISA Kit, Colorimetric assay for quantification of Mouse IL15RA.

Quick Overview for IL15RA ELISA Kit (ABIN6720034)

Target: IL15RA (Interleukin 15 Receptor, alpha (IL15RA))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 125 pg/mL - 8000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details IL15RA ELISA Kit

Minimum Detection Limit: 125 pg/mL

Supplier Product No.: koa0654

Supplier: Rockland

Purpose: Mouse IL-15RA Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: G33-K205

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse IL15RA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse IL15RA standard solution and each sample be measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 6.9%, 6.9%, 5%
Inter-Assay Precision (CV%): 7%, 8.4%, 5%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for IL15RA

Target: IL15RA (Interleukin 15 Receptor, alpha (IL15RA))

Alternative Name: IL15RA

Background:

Background: Interleukin 15 receptor, alpha subunit is a subunit of the interleukin 15 receptor which is encoded by the gene IL15RA in humans. The human IL15RA gene was mapped to chromosome 10p15-p14 by FISH and the mouse Il15ra gene was mapped to chromosome 2. The cytoplasmic domain of IL15R-alpha, like that of IL2R-alpha, was dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high-affinity binding. At high concentrations, IL15, like IL2, was able to signal through a complex of IL2R-beta and -gamma in the absence of the alpha subunit. The soluble receptor displayed high affinity for IL15 and inhibited both binding of IL15 to the membrane receptor and IL15-induced cell proliferation.

Gene Full Name: interleukin 15 receptor, alpha chain

Gene ID: 16169

NCBI Accession: NP_001258426

UniProt: Q60819

Pathways: JAK-STAT Signaling