CRP ELISA Kit

Catalog No. ABIN6720138

Supplier Product No.: koa0606

The Rat CRP ELISA Kit (ABIN6720138) is a Colorimetric ELISA Kit designed to quantify Rat CRP.

Quick Overview for CRP ELISA Kit (ABIN6720138)

Target: CRP (C-Reactive Protein (CRP))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details CRP ELISA Kit

Minimum Detection Limit: 156 pg/mL

Supplier Product No.: koa0606

Supplier: Rockland

Purpose: Rat CRP Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: H20-S230

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat CRP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat CRP standard solution and each sample be measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 4.9%, 5.4%, 6.4%
Inter-Assay Precision (CV%): 5%, 7.2%, 7.4%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CRP

Target: CRP (C-Reactive Protein (CRP))

Alternative Name: CRP

Background:

Background: C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4 % of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Gene Full Name: C-reactive protein

Gene ID: 25419

NCBI Accession: NP_058792

UniProt: P48199

Pathways: Carbohydrate Homeostasis