IL-5 ELISA Kit

Catalog No. ABIN6720357

Product Details IL-5 ELISA Kit

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8 pg/mL - 500 pg/mL

Minimum Detection Limit: 7.8 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine

Analytical Method: Quantitative

Characteristics:

Synonyms: B cell differentiation factor I, B-cell differentiation factor I, Colony stimulating factor eosinophil, EDF, Eosinophil differentiation factor, IL 5, IL-5, IL5, IL5_HUMAN, Interleukin 5, Interleukin 5 (colony stimulating factor eosinophil), Interleukin-5 Interleukin5, T cell replacing factor, T-cell replacing factor, TRF

Background: Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70 % amino acid sequence homology.

Gene Name: IL5

Production: Natural and recombinant human IL-5. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: SF21, Immunogen sequence: I20-S134

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human IL-5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each human IL-5 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 7.8pg/mL-500pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: IL-5 (IL5 Products)

Synonyms: EDF ELISA Kit, IL-5 ELISA Kit, TRF ELISA Kit, Il-5 ELISA Kit, IL5 ELISA Kit, interleukin 5 ELISA Kit, IL5 ELISA Kit, Il5 ELISA Kit

Gene ID: 3567

NCBI Accession: NP_000870

UniProt: P05113

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour