Klotho beta ELISA Kit

Catalog No. ABIN6730087

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse Klotho beta.

Quick Overview for Klotho beta ELISA Kit (ABIN6730087)

Target: Klotho beta (KLB)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.6 ng/mL - 500 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details Klotho beta ELISA Kit

Minimum Detection Limit: 1.6 ng/mL

Purpose: Mouse Klotho beta ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse ADAM9, CD14, CD39L3, CDNF, Cripto, CXCL14, Epimorphin, Erythropoietin R, Flt-3, Follistatin, Frizzled-1, Frizzled-4, FSTL1, GDF-3, GDF-7, GFR alpha-3, IFN-gamma R2, IL-1 RI, IL-1 RII, IL-10 R beta, IL-11 R alpha, IL-15 R alpha, IL-17 RA, IL-17 RC, IL-20 R beta, IL-1F8, IL-21 R, IL23 R, IL-28A, JAM-C, Layilin, LDL R, LIF, Matrilin-3, Lymphotoxin beta R, Nephrin, Neurocan, NKp46, Laminin alpha 4.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Suggested Plasma Dilution: 2 fold

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Klotho beta

Target: Klotho beta (KLB)

Alternative Name: Klotho beta

Background:

Gene Symbols: BETAKL, KLB

Protein Name (Synonyms): Beta-klotho (BKL) (BetaKlotho) (Klotho beta-like protein)

UniProt: Q99N32

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Growth Factor Binding