Nicastrin ELISA Kit

Catalog No. ABIN6730247

Product Details Nicastrin ELISA Kit

Target: Nicastrin (NCSTN)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse Nicastrin/NCSTN ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse Nicastrin.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Nicastrin

Target: Nicastrin (NCSTN)

Alternative Name: Nicastrin (NCSTN (NCSTN Products)

Synonyms: CG7012 ELISA Kit, Dmel\\CG7012 ELISA Kit, Nct ELISA Kit, Nic ELISA Kit, S5A ELISA Kit, ST96B ELISA Kit, agro ELISA Kit, dNct ELISA Kit, nic ELISA Kit, NCSTN ELISA Kit, DKFZp469O2418 ELISA Kit, APH2 ELISA Kit, 9430068N19Rik ELISA Kit, AA727311 ELISA Kit, Aph2 ELISA Kit, D1Dau13e ELISA Kit, Kiaa0253 ELISA Kit, mKIAA0253 ELISA Kit, nicastrin ELISA Kit, Nicastrin ELISA Kit, NCSTN ELISA Kit, nct ELISA Kit, ncstn ELISA Kit, CpipJ_CPIJ001617 ELISA Kit, Ncstn ELISA Kit, aph-2 ELISA Kit

UniProt: P57716

Pathways: Notch Signaling, Neurotrophin Signaling Pathway