CSNK2A1/CK II alpha ELISA Kit

Catalog No. ABIN6730466

Product Details CSNK2A1/CK II alpha ELISA Kit

Target: CSNK2A1/CK II alpha (CSNK2A1) (Casein Kinase 2 alpha 1 (CSNK2A1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse CSNK2A1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse CSNK2A1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CSNK2A1/CK II alpha

Target: CSNK2A1/CK II alpha (CSNK2A1) (Casein Kinase 2 alpha 1 (CSNK2A1))

Alternative Name: CSNK2A1 (CSNK2A1 Products)

Synonyms: CK2A2 ELISA Kit, CSNK2A1 ELISA Kit, CK2A1 ELISA Kit, CKII ELISA Kit, CSNK2A3 ELISA Kit, CK2A ELISA Kit, Ck2a ELISA Kit, BmCK2a ELISA Kit, wu:fi38e04 ELISA Kit, wu:fi38h03 ELISA Kit, csnk2a1 ELISA Kit, CK-II ELISA Kit, CK2 ELISA Kit, Ckiialpha ELISA Kit, Csnk2a1-rs4 ELISA Kit, ck2a1 ELISA Kit, casein kinase 2 alpha 2 ELISA Kit, casein kinase 2 alpha 1 ELISA Kit, casein kinase 2 alpha subunit ELISA Kit, casein kinase 2, alpha 1 polypeptide ELISA Kit, CK2 protein kinase alpha 2 ELISA Kit, casein kinase II alpha subunit ELISA Kit, Casein kinase II alpha subunit ELISA Kit, casein kinase 2, alpha 1 polypeptide S homeolog ELISA Kit, CSNK2A2 ELISA Kit, CSNK2A1 ELISA Kit, ck2a ELISA Kit, Ck2a ELISA Kit, csnk2a1 ELISA Kit, cka2 ELISA Kit, Ckiialpha ELISA Kit, CK2A1 ELISA Kit, Csnk2a1 ELISA Kit, csnk2a1.S ELISA Kit

UniProt: Q60737

Pathways: SARS-CoV-2 Protein Interactome