PTK2B ELISA Kit

Catalog No. ABIN6730589

Product Details PTK2B ELISA Kit

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human PYK2/FAK2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human PYK2.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for PTK2B

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Alternative Name: PYK2 (FAK2 (PTK2B Products)

Synonyms: CADTK ELISA Kit, CAKB ELISA Kit, FADK2 ELISA Kit, FAK2 ELISA Kit, PKB ELISA Kit, PTK ELISA Kit, PYK2 ELISA Kit, RAFTK ELISA Kit, CAKbeta ELISA Kit, E430023O05Rik ELISA Kit, Raftk ELISA Kit, Pyk2 ELISA Kit, fak1b ELISA Kit, ptk2.2 ELISA Kit, ptk2b ELISA Kit, si:ch1073-355e8.1 ELISA Kit, protein tyrosine kinase 2 beta ELISA Kit, PTK2 protein tyrosine kinase 2 beta ELISA Kit, protein tyrosine kinase 2aa ELISA Kit, PTK2B ELISA Kit, Ptk2b ELISA Kit, ptk2aa ELISA Kit

UniProt: Q14289

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling