PTK2B ELISA Kit (PTK2B Protein tyrosine Kinase 2 beta)

Details for Product PTK2B ELISA Kit No. ABIN6730589
  • CAKB
  • FADK2
  • FAK2
  • PKB
  • PTK
  • PYK2
  • CAKbeta
  • E430023O05Rik
  • Raftk
  • Pyk2
  • fak1b
  • ptk2.2
  • ptk2b
  • si:ch1073-355e8.1
  • protein tyrosine kinase 2 beta
  • PTK2 protein tyrosine kinase 2 beta
  • protein tyrosine kinase 2aa
  • PTK2B
  • Ptk2b
  • ptk2aa
Kits with alternative reactivity to:
Detection Method
Method Type
Sandwich ELISA
Purpose Human PYK2/FAK2 ELISA Kit.
Sample Type Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This ELISA antibody pair recognizes Human PYK2.
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Plasmids, Primers & others Plasmids, Primers & others PTK2B products on genomics-online (e.g. as negative or positive controls)
Alternative Name PYK2 (FAK2 (PTK2B ELISA Kit Abstract)
UniProt Q14289
Pathways EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling
Application Notes Optimal working dilution should be determined by the investigator.
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months
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