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CSF1R ELISA Kit
CSF1R
Reactivity: Mouse
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Product Details CSF1R ELISA Kit
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Target
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CSF1R
(Colony Stimulating Factor 1 Receptor (CSF1R))
Reactivity
All reactivities for CSF1R elisa kits
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Mouse M-CSF R/CD115 ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes Mouse M-CSF R.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for CSF1R
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Target
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CSF1R
(Colony Stimulating Factor 1 Receptor (CSF1R))
Alternative Name
M-CSF R (CD115 (CSF1R Products )
Synonyms
AI323359 ELISA Kit, CD115 ELISA Kit, CSF-1R ELISA Kit, Csfmr ELISA Kit, Fim-2 ELISA Kit, Fms ELISA Kit, M-CSF-R ELISA Kit, M-CSFR ELISA Kit, C-FMS ELISA Kit, CSFR ELISA Kit, FIM2 ELISA Kit, FMS ELISA Kit, HDLS ELISA Kit, PDGFRB ELISA Kit, MGC89146 ELISA Kit, CSF-1-R ELISA Kit, c-fms ELISA Kit, mrfms ELISA Kit, CSF1 ELISA Kit, M-CSF ELISA Kit, csf1r ELISA Kit, fms ELISA Kit, panther ELISA Kit, colony stimulating factor 1 receptor ELISA Kit, macrophage colony-stimulating factor receptor ELISA Kit, colony stimulating factor 1 receptor, a ELISA Kit, Csf1r ELISA Kit, CSF1R ELISA Kit, m-csfr ELISA Kit, csf1r ELISA Kit, csf1ra ELISA Kit
UniProt
P09581
Pathways
RTK Signaling , Inositol Metabolic Process , Cell-Cell Junction Organization
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