GLA ELISA Kit

Catalog No. ABIN6730640

Product Details GLA ELISA Kit

Target: GLA (Galactosidase, alpha (GLA))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse GLA ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse GLA.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for GLA

Target: GLA (Galactosidase, alpha (GLA))

Alternative Name: GLA (GLA Products)

Synonyms: GALA ELISA Kit, Ags ELISA Kit, zgc:101584 ELISA Kit, MGC130872 ELISA Kit, SMU.877 ELISA Kit, SCF11.21 ELISA Kit, AO090005000217 ELISA Kit, alpha-GAL ELISA Kit, galactosidase alpha ELISA Kit, galactosidase, alpha ELISA Kit, galactosidase alpha S homeolog ELISA Kit, alpha-galactosidase ELISA Kit, aga ELISA Kit, alpha-galactosidase A ELISA Kit, GLA ELISA Kit, Gla ELISA Kit, gla ELISA Kit, gla.S ELISA Kit, agaN ELISA Kit, aga ELISA Kit, agaL ELISA Kit, SCO0541 ELISA Kit, rafA ELISA Kit, melA ELISA Kit, galA ELISA Kit, ANI_1_2528074 ELISA Kit, ANI_1_1502124 ELISA Kit, AOR_1_390174 ELISA Kit, CpipJ_CPIJ002066 ELISA Kit, MCYG_00962 ELISA Kit, MCYG_00791 ELISA Kit, Tsp_02909 ELISA Kit, Tsp_02508 ELISA Kit

UniProt: Q8BGZ6

Pathways: SARS-CoV-2 Protein Interactome