CHD7 ELISA Kit

Catalog No. ABIN6951098

Product Details CHD7 ELISA Kit

Target: CHD7 (Chromodomain Helicase DNA Binding Protein 7 (CHD7))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human CHD7 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for CHD7

Target: CHD7 (Chromodomain Helicase DNA Binding Protein 7 (CHD7))

Alternative Name: CHD7 (CHD7 Products)

Synonyms: CHD7 ELISA Kit, fd19h06 ELISA Kit, si:ch211-197o6.2 ELISA Kit, wu:cegs2051 ELISA Kit, wu:fb37f10 ELISA Kit, wu:fb39h04 ELISA Kit, wu:fd19h06 ELISA Kit, HH5 ELISA Kit, IS3 ELISA Kit, KAL5 ELISA Kit, A730019I05Rik ELISA Kit, Cycn ELISA Kit, Cyn ELISA Kit, Dz ELISA Kit, Edy ELISA Kit, Flo ELISA Kit, GENA 47 ELISA Kit, GENA 60 ELISA Kit, Gena 52 ELISA Kit, Lda ELISA Kit, Mt ELISA Kit, Obt ELISA Kit, Todo ELISA Kit, WBE1 ELISA Kit, Whi ELISA Kit, metis ELISA Kit, chromodomain helicase DNA binding protein 7 L homeolog ELISA Kit, chromodomain helicase DNA binding protein 7 ELISA Kit, chd7.L ELISA Kit, CHD7 ELISA Kit, chd7 ELISA Kit, Chd7 ELISA Kit

Gene ID: 55636

UniProt: Q9P2D1

Pathways: Sensory Perception of Sound, Chromatin Binding