Ephrin A1 ELISA Kit

Catalog No. ABIN6951120

Product Details Ephrin A1 ELISA Kit

Target: Ephrin A1 (EFNA1)

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Rat EFNA1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for Ephrin A1

Target: Ephrin A1 (EFNA1)

Alternative Name: EFNA1 (EFNA1 Products)

Synonyms: Xefrin-A1 ELISA Kit, b61 ELISA Kit, ecklg ELISA Kit, efl1 ELISA Kit, efna1 ELISA Kit, efna1a ELISA Kit, elfa-a ELISA Kit, epha1 ELISA Kit, eplg1 ELISA Kit, lerk1 ELISA Kit, tnfaip4 ELISA Kit, xelf-a' ELISA Kit, xelfa ELISA Kit, AI325262 ELISA Kit, B61 ELISA Kit, Efl1 ELISA Kit, Epl1 ELISA Kit, Eplg1 ELISA Kit, Lerk1 ELISA Kit, ECKLG ELISA Kit, EFL1 ELISA Kit, EPLG1 ELISA Kit, LERK-1 ELISA Kit, LERK1 ELISA Kit, TNFAIP4 ELISA Kit, efna1b ELISA Kit, MGC130661 ELISA Kit, ephrin-A1 S homeolog ELISA Kit, ephrin-A1 ELISA Kit, ephrin A1 ELISA Kit, ephrin-A1b ELISA Kit, ephrin-A1 L homeolog ELISA Kit, efna1.S ELISA Kit, efna1 ELISA Kit, EFNA1 ELISA Kit, efna1b ELISA Kit, Efna1 ELISA Kit, efna1.L ELISA Kit

Gene ID: 94268

UniProt: P97553

Pathways: RTK Signaling