RBP4 ELISA Kit
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- Target See all RBP4 ELISA Kits
- RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
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Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Rat RBP4 ELISA Kit.
- Sample Type
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product RBP4 ELISA Kit
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Expiry Date
- 6 months
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- Target See all RBP4 ELISA Kits
- RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
- Alternative Name
- RBP4 (RBP4 Products)
- Synonyms
- RDCCAS ELISA Kit, RBP ELISA Kit, RNBP ELISA Kit, rbp4 ELISA Kit, MGC54038 ELISA Kit, srbp ELISA Kit, PRBP ELISA Kit, RBPA ELISA Kit, Rbp-4 ELISA Kit, fb23c12 ELISA Kit, rbp ELISA Kit, wu:fb23c12 ELISA Kit, wu:fb58d04 ELISA Kit, wu:fb72b04 ELISA Kit, zgc:86911 ELISA Kit, retinol binding protein 4 ELISA Kit, renin binding protein ELISA Kit, retinol binding protein 4 S homeolog ELISA Kit, retinol binding protein 4 A, plasma ELISA Kit, retinol binding protein 4, plasma ELISA Kit, retinol binding protein 4 L homeolog ELISA Kit, RBP4 ELISA Kit, RENBP ELISA Kit, rbp4.S ELISA Kit, rbp4 ELISA Kit, Rbp4 ELISA Kit, RBP4A ELISA Kit, rbp4.L ELISA Kit
- Gene ID
- 25703
- UniProt
- P04916
- Pathways
- Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response
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