RAB7A ELISA Kit

Catalog No. ABIN6951224

Product Details RAB7A ELISA Kit

Target: RAB7A (RAB7A, Member RAS Oncogene Family (RAB7A))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Rat RAB7A ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for RAB7A

Target: RAB7A (RAB7A, Member RAS Oncogene Family (RAB7A))

Alternative Name: RAB7A (RAB7A Products)

Synonyms: RAB5 ELISA Kit, PRO2706 ELISA Kit, RAB7 ELISA Kit, Rab7 ELISA Kit, rab7 ELISA Kit, pro2706 ELISA Kit, RAB7A ELISA Kit, LOC100231734 ELISA Kit, ARABIDOPSIS RAB GTPASE HOMOLOG G2 ELISA Kit, ATRAB7A ELISA Kit, ATRABG2 ELISA Kit, F7D8.20 ELISA Kit, F7D8_20 ELISA Kit, RAB GTPase homolog 7A ELISA Kit, DDBDRAFT_0190210 ELISA Kit, DDBDRAFT_0191507 ELISA Kit, DDB_0190210 ELISA Kit, DDB_0191507 ELISA Kit, Rab7a ELISA Kit, rab-7 ELISA Kit, zgc:64058 ELISA Kit, RAB5A, member RAS oncogene family ELISA Kit, RAB7A, member RAS oncogene family ELISA Kit, RAB7A, member RAS oncogene family L homeolog ELISA Kit, rab7A protein ELISA Kit, RAB GTPase homolog 7A ELISA Kit, Rab GTPase ELISA Kit, ras-related protein rab-7a ELISA Kit, RAB7, member RAS oncogene family ELISA Kit, RAB5A ELISA Kit, RAB7A ELISA Kit, Rab7a ELISA Kit, rab7a.L ELISA Kit, rab7a ELISA Kit, rab7A ELISA Kit, Rab7 ELISA Kit, rab7 ELISA Kit

Gene ID: 29448

UniProt: P09527

Pathways: EGFR Signaling Pathway, Maintenance of Protein Location, SARS-CoV-2 Protein Interactome