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DREAM ELISA Kit

KCNIP3 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN6951435
  • Target See all DREAM (KCNIP3) ELISA Kits
    DREAM (KCNIP3) (Kv Channel Interacting Protein 3, Calsenilin (KCNIP3))
    Reactivity
    • 2
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human KCNIP3 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product KCNIP3 ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Expiry Date
    6 months
  • Target See all DREAM (KCNIP3) ELISA Kits
    DREAM (KCNIP3) (Kv Channel Interacting Protein 3, Calsenilin (KCNIP3))
    Alternative Name
    KCNIP3 (KCNIP3 Products)
    Synonyms
    CSEN ELISA Kit, DREAM ELISA Kit, KCHIP3 ELISA Kit, 4933407H12Rik ELISA Kit, AI413860 ELISA Kit, Csen ELISA Kit, KChIP3 ELISA Kit, R74849 ELISA Kit, Dream ELISA Kit, rKChIP3 ELISA Kit, csen ELISA Kit, im:7145308 ELISA Kit, kcnip3 ELISA Kit, zgc:73306 ELISA Kit, KCNIP3 ELISA Kit, potassium voltage-gated channel interacting protein 3 ELISA Kit, Kv channel-interacting protein 4 ELISA Kit, calsenilin ELISA Kit, Kv channel interacting protein 3, calsenilin ELISA Kit, Kv channel interacting protein 3b, calsenilin ELISA Kit, KCNIP3 ELISA Kit, LOC5568664 ELISA Kit, CpipJ_CPIJ002549 ELISA Kit, Tsp_05009 ELISA Kit, Kcnip3 ELISA Kit, kcnip3b ELISA Kit, kcnip3 ELISA Kit
    Gene ID
    30818
    UniProt
    Q9Y2W7
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