ATG13 ELISA Kit

Catalog No. ABIN6951729

Product Details ATG13 ELISA Kit

Target: ATG13 (Autophagy Related 13 (ATG13))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ATG13 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for ATG13

Target: ATG13 (Autophagy Related 13 (ATG13))

Alternative Name: ATG13 (ATG13 Products)

Synonyms: zgc:63526 ELISA Kit, KIAA0652 ELISA Kit, PARATARG8 ELISA Kit, 1110053A20Rik ELISA Kit, D2Ertd391e ELISA Kit, Harbi1 ELISA Kit, Harbi1l ELISA Kit, RGD1310685 ELISA Kit, autophagy related 13 ELISA Kit, ATG13 autophagy related 13 homolog (S. cerevisiae) ELISA Kit, ATG13 ELISA Kit, atg13 ELISA Kit, Atg13 ELISA Kit

Gene ID: 9776

UniProt: O75143

Pathways: PI3K-Akt Signaling, Autophagy