SARS-CoV-2 N-Protein IgG Antibody ELISA Kit

Catalog No. ABIN6952759

The Human SARS-CoV-2 N-Protein IgG Antibody ELISA Kit (ABIN6952759) is a Colorimetric ELISA Kit designed to quantify Human SARS-CoV-2 N-Protein IgG Antibody.

Quick Overview for SARS-CoV-2 N-Protein IgG Antibody ELISA Kit (ABIN6952759)

Target: SARS-CoV-2 N-Protein IgG Antibody

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Dried Blood, Plasma, Saliva, Serum

Product Details

Purpose: COVID-19 N and S1 RBD protein Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgG antibody against SARS-CoV-2 in serum. This Product is for Research Use Only. Not for use in in vitro diagnostics.

Analytical Method: Semi-Quantitative

Characteristics: COVID-19 N-Protein Human IgG ELISA Kit

Components:

• SARS-CoV-2 N-protein coated 96-well microplate
• Wash Buffer
• Positive Control
• Biotinylated Anti-Human IgG
• HRP-Streptavidin concentrate
• TMB One-Step Substrate
• Stop Solution
• Assay Diluent
• 5X Sample Diluent
• 1X PBS (DBS version)
• Tween-20 (DBS version)
• Protease Inhibitor Cocktail (DBS version)

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Distilled or deionized water

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 μL prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
4. Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Assay Procedure:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 1.5 hours at room temperature.
3. Add 100 μL prepared HRP- anti positive control IgG Antibody and/or 100 μL of prepared HRP-anti Human IgG Antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
5. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
6. Read at 450 nm immediately.

Calculation of Results:

Calibration curve: Calculate the mean absorbance for each set of duplicate Positive Control (Item C), samples, and subtract the average zero Positive Control optical density. Plot the calibration curve on log-log with Positive Control concentration (Unit/mL) on the xaxis and absorbance on the y-axis using Sigma plot or Excel software.

Restrictions: For Research Use only

Handling

Storage: -80 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 N-Protein IgG Antibody

Target Type: Antibody

Background: Gene Symbols: IGG