CDK1 ELISA Kit

Catalog No. ABIN7830490

Human CDK1 ELISA Kit, Colorimetric assay for quantification of Human CDK1.

Quick Overview for CDK1 ELISA Kit (ABIN7830490)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details CDK1 ELISA Kit

Purpose: CDC2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CDC2 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: CDC2

Background:

Synonyms: CDC28A|CDK1|DKFZp686L20222|MGC111195

Gene Description: cell division cycle 2, G1 to S and G2 to M

Gene ID: 983

UniProt: P06493

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA