CDK7 ELISA Kit

Catalog No. ABIN7830496

Human CDK7 ELISA Kit, Colorimetric assay for quantification of Human CDK7.

Quick Overview for CDK7 ELISA Kit (ABIN7830496)

Target: CDK7 (Cyclin-Dependent Kinase 7 (CDK7))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details CDK7 ELISA Kit

Purpose: CDK7 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CDK7 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for CDK7

Target: CDK7 (Cyclin-Dependent Kinase 7 (CDK7))

Alternative Name: CDK7

Background:

Synonyms: CAK1|CDKN7|MO15|STK1|p39MO15

Gene Description: cyclin-dependent kinase 7

Gene ID: 1022

UniProt: P50613

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, M Phase