E2F1 ELISA Kit

Catalog No. ABIN7830610

Human E2F1 ELISA Kit, Colorimetric assay for quantification of Human E2F1.

Quick Overview for E2F1 ELISA Kit (ABIN7830610)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details E2F1 ELISA Kit

Purpose: E2F1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of E2F1 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background:

Synonyms: E2F-1|RBAP1|RBBP3|RBP3

Gene Description: E2F transcription factor 1

Gene ID: 1869

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy