EPH Receptor B2 ELISA Kit

Catalog No. ABIN7830629

This Colorimetric ELISA kit is designed for the quantitative measurement of Human EPH Receptor B2.

Quick Overview for EPH Receptor B2 ELISA Kit (ABIN7830629)

Target: EPH Receptor B2 (EPHB2)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details EPH Receptor B2 ELISA Kit

Purpose: EPHB2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of EPHB2 expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for EPH Receptor B2

Target: EPH Receptor B2 (EPHB2)

Alternative Name: EPHB2

Background:

Synonyms: CAPB|DRT|EPHT3|ERK|Hek5|MGC87492|PCBC|Tyro5

Gene Description: EPH receptor B2

Gene ID: 2048

UniProt: P29323

Pathways: RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins