GATA4 ELISA Kit

Catalog No. ABIN7830673

Human GATA4 ELISA Kit, Colorimetric assay for quantification of Human GATA4.

Quick Overview for GATA4 ELISA Kit (ABIN7830673)

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details GATA4 ELISA Kit

Purpose: GATA4 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of GATA4 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for GATA4

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Alternative Name: GATA4

Background:

Synonyms: MGC126629

Gene Description: GATA binding protein 4

Gene ID: 2626

UniProt: P43694

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis