JAK2 ELISA Kit

Catalog No. ABIN7830713

This Colorimetric ELISA kit is designed for the quantitative measurement of Human JAK2.

Quick Overview for JAK2 ELISA Kit (ABIN7830713)

Target: JAK2 (Janus Kinase 2 (JAK2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details JAK2 ELISA Kit

Purpose: JAK2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of JAK2 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for JAK2

Target: JAK2 (Janus Kinase 2 (JAK2))

Alternative Name: JAK2

Background:

Synonyms: JTK10

Gene Description: Janus kinase 2 (a protein tyrosine kinase)

Gene ID: 3717

UniProt: O60674

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response