p300 ELISA Kit

Catalog No. ABIN7830797

This Colorimetric ELISA kit is designed for the quantitative measurement of Human p300.

Quick Overview for p300 ELISA Kit (ABIN7830797)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details p300 ELISA Kit

Purpose: EP300 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of EP300 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: EP300

Background:

Synonyms: KAT3B|p300

Gene Description: E1A binding protein p300

Gene ID: 2033

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size