MTOR ELISA Kit
Quick Overview for MTOR ELISA Kit (ABIN7830800)
Target
See all MTOR (mTOR) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- MTOR (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MTOR expression in cultured cells.
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Analytical Method
- Qualitative
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Cross-Reactivity
- Mouse, Rat
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Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA Kit
mTOR Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA KitmTOR Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA KitmTOR Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA KitmTOR Reactivity: Human Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA KitmTOR Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA KitmTOR Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
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Alternative Name
- MTOR
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Background
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Synonyms: FRAP|FRAP1|FRAP2|RAFT1|RAPT1
Gene Description: mechanistic target of rapamycin
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Gene ID
- 2475
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Pathways
- PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect
Target See all MTOR (mTOR) ELISA Kits
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