INPP5D ELISA Kit

Catalog No. ABIN7830805

Human INPP5D ELISA Kit, Colorimetric assay for quantification of Human INPP5D.

Quick Overview for INPP5D ELISA Kit (ABIN7830805)

Target: INPP5D (Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details INPP5D ELISA Kit

Purpose: INPP5D (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of INPP5D expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for INPP5D

Target: INPP5D (Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D))

Alternative Name: INPP5D

Background:

Synonyms: MGC104855|MGC142140|MGC142142|SHIP|SHIP1|SIP-145|hp51CN

Gene Description: inositol polyphosphate-5-phosphatase, 145 kDa

Gene ID: 3635

Pathways: TCR Signaling, BCR Signaling, Warburg Effect