PPARG ELISA Kit
Quick Overview for PPARG ELISA Kit (ABIN7830849)
Target
See all PPARG ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- PPARG (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PPARG expression in cultured cells.
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Analytical Method
- Qualitative
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Cross-Reactivity
- Mouse, Rat
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Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA Kit
PPARG Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Rabbit Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Human Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Rat Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))
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Alternative Name
- PPARG
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Background
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Synonyms: CIMT1|NR1C3|PPARG1|PPARG2|PPARgamma
Gene Description: peroxisome proliferator-activated receptor gamma
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Gene ID
- 5468
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Pathways
- MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
Target See all PPARG ELISA Kits
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