STAT5B ELISA Kit

Catalog No. ABIN7830905

This Colorimetric ELISA kit is designed for the quantitative measurement of Human STAT5B.

Quick Overview for STAT5B ELISA Kit (ABIN7830905)

Target: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details STAT5B ELISA Kit

Purpose: STAT5B (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of STAT5B expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for STAT5B

Target: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Alternative Name: STAT5B

Background:

Synonyms: STAT5

Gene Description: signal transducer and activator of transcription 5B

Gene ID: 6777

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation