WFDC2 ELISA Kit
Quick Overview for WFDC2 ELISA Kit (ABIN7832864)
Target
See all WFDC2 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
Grade
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Purpose
- FastELISA WFDC2 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human WFDC2 (Human epididymis protein 4, HE4) in Serum, plasma.
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Analytical Method
- Quantitative
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Specificity
- This assay is highly specific for detection of Human WFDC2. No significant cross-reactivity or interference between Human WFDC2 and analogues was observed.
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WAP Four-Disulfide Core Domain 2 (WFDC2) ELISA Kit
WFDC2 Reactivity: Human Colorimetric Sandwich ELISA 0.78 pmol/mL - 50 pmol/mL Cell Culture Supernatant, Plasma, Serum
WAP Four-Disulfide Core Domain 2 (WFDC2) ELISA KitWFDC2 Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Saliva, Serum, Tissue Homogenate
WAP Four-Disulfide Core Domain 2 (WFDC2) ELISA KitWFDC2 Reactivity: Mouse Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
WAP Four-Disulfide Core Domain 2 (WFDC2) ELISA KitWFDC2 Reactivity: Human AA 31-124 Colorimetric Sandwich ELISA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freezing and thawing
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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Expiry Date
- 6 months
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- WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))
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Alternative Name
- WFDC2
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Background
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Synonyms: HE4|MGC57529|WAP5|dJ461P17.6
Gene Description: WAP four-disulfide core domain 2
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Gene ID
- 10406
Target See all WFDC2 ELISA Kits
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