Lipocalin 2 ELISA Kit
Quick Overview for Lipocalin 2 ELISA Kit (ABIN7832871)
Target
See all Lipocalin 2 (LCN2) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
Grade
-
-
Purpose
- FastELISA LCN2 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human LCN2 (Neutrophil gelatinase-associated lipocalin, NGAL) in Serum, plasma.
-
Analytical Method
- Quantitative
-
Specificity
- This assay is highly specific for detection of Human LCN2. No significant cross-reactivity or interference between Human LCN2 and analogues was observed.
-
-
Lipocalin 2 (LCN2) ELISA Kit
LCN2 Reactivity: Human Colorimetric Sandwich ELISA 15.6-1000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Human Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Mouse Colorimetric Competition ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Rat Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Human AA 21-198 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Saliva, Serum, Urine
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Rat AA 21-198 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum, Urine
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Rat Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Plate
- Pre-coated
-
Protocol
-
- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
-
Restrictions
- For Research Use only
-
-
-
Handling Advice
- Avoid repeated freezing and thawing
-
Storage
- 4 °C,-20 °C
-
Storage Comment
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
-
Expiry Date
- 6 months
-
-
- Lipocalin 2 (LCN2)
-
Alternative Name
- LCN2
-
Background
-
Synonyms: NGAL
Gene Description: lipocalin 2
-
Gene ID
- 3934
-
Pathways
- Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis
Target See all Lipocalin 2 (LCN2) ELISA Kits
-
