VCAM1 ELISA Kit
Quick Overview for VCAM1 ELISA Kit (ABIN7832883)
Target
See all VCAM1 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
Grade
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Purpose
- FastELISA VCAM1 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human VCAM1 (Vascular Cell Adhesion Molecule 1) in Serum, plasma.
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Analytical Method
- Quantitative
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Specificity
- This assay is highly specific for detection of Human VCAM1. No significant cross-reactivity or interference between Human VCAM1 and analogues was observed.
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Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit
VCAM1 Reactivity: Human Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reactivity: Mouse Colorimetric Sandwich ELISA 39.062-2500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reactivity: Rat Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reactivity: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reactivity: Rabbit Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reactivity: Pig Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reactivity: Human AA 25-698 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reactivity: Dog Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reactivity: Monkey Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freezing and thawing
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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Expiry Date
- 6 months
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- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
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Alternative Name
- VCAM1
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Background
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Synonyms: CD106|DKFZp779G2333|INCAM-100|MGC99561
Gene Description: vascular cell adhesion molecule 1
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Gene ID
- 7412
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Pathways
- Carbohydrate Homeostasis
Target See all VCAM1 ELISA Kits
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