VCAM1 ELISA Kit

Catalog No. ABIN7964547

Mouse VCAM1 ELISA Kit, Colorimetric assay for quantification of Mouse VCAM1.

Quick Overview for VCAM1 ELISA Kit (ABIN7964547)

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.13 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Biological Fluids, Plasma, Serum

Grade: Quick

Product Details VCAM1 ELISA Kit

Minimum Detection Limit: 78.13 pg/mL

Purpose: Quick Mouse VCAM-1/CD106 (Vascular cell adhesion molecule 1) ELISA Kit

Analytical Method: Quantitative

Cross-Reactivity (Details): This kit recognizes Mouse VCAM-1/CD106 in samples.No significant cross-reactivity or interference between and analogues was observed

Sensitivity: 46.88 pg/mL

Components:

• Precoated Micro ELISA Plate, Dismountable
• Reference Standard
• Concentrated HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• HRP Conjugate Diluent
• Concentrated Wash Buffer (25x)
• Substrate Reagent
• Stop Solution
• Plate Sealer
• Manual

Note: Reagents may contain slightly more volume than indicated on the label; mix and centrifuge the vial of reagents before use. Use precise measuring instruments rather than pouring directly from the vial.

Application Details

Sample Volume: 50 μL

Assay Time: 1.5 h

Plate: Pre-coated

Protocol:

1. Add 50 µL standard or sample to each well.
2. Add 50 µL HRP Conjugate. Incubate for 60 min at 37 °C.
3. Aspirate and wash 5 times.
4. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
5. Add 50 µL Stop Solution. Read at 450 nm immediately.
6. Calculation of results.

Reagent Preparation:

1. Bring all reagents to room temperature (18 - 25 °C) before use.
2. Standard Working Solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard & Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution, then make serial dilutions as needed. Note: Serially diluted standards should be prepared immediately, prior to use.
3. HRP Conjugate Working Solution: Calculate the required amount before the experiment. When preparing, prepare slightly more than calculated. Centrifuge the Concentrated HRP Conjugate at 800×g for 1 min, then dilute the 100× Concentrated HRP Conjugate to 1× working solution with HRP Conjugate Diluent. Note: Prepare the working solution immediately, prior to use.
4. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer. Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved.

Sample Preparation:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.

Assay Precision:

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level of the target were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level of the target were tested on 3 different plates, 20 replicates in each plate.

Both intra-CV and inter-CV are < 10%.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

• For unopened kit: All reagents should be stored according to the labels on the vials. Protect Concentrated HRP Conjugate and Substrate Reagent from light. Always use the reconstituted standard within 24h. Reconstituted standard may be stored aliquoted at -20 °C for up to two weeks. Store samples at – 80 °C for up to 3 months. Avoid repeated freeze-thaw cycles.
• For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Alternative Name: VCAM1

Background:

Target Synonym: VCAM1,CD106,INCAM-100,vascular cell adhesion molecule 1

Background: Vascular cell adhesion molecule-1 (VCAM-1, CD106) is a type I membrane glycoprotein of the immunoglobulin superfamily. Mouse VCAM-1 has two isoforms: a 110 kDa transmembrane long isoform and a 45 kDa GPI-anchored short isoform, both releasable as soluble

Gene ID: 22329

UniProt: P29533

Pathways: Carbohydrate Homeostasis