EPH Receptor A4 ELISA Kit

Catalog No. ABIN8055667

The Mouse EPH Receptor A4 ELISA Kit (ABIN8055667) is a Colorimetric ELISA Kit designed to quantify Mouse EPH Receptor A4.

Quick Overview for EPH Receptor A4 ELISA Kit (ABIN8055667)

Target: EPH Receptor A4 (EPHA4)

Binding Specificity: AA 20-547

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Product Details EPH Receptor A4 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich Mouse EphA4 ELISA Kit to quantitate Mouse Epha4 in cell culture supernatants, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: V20-T547

Capture antibody and Detection antibody: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 5.8%, 6.0%, 4.6%
Inter-Assay Precision (CV%): 6.1%, 6.9%, 7.3%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

Target Details for EPH Receptor A4

Target: EPH Receptor A4 (EPHA4)

Alternative Name: Epha4

Background:

Background: EPH receptor A4 (ephrin type-A receptor 4) is a protein that in humans is encoded by the EPHA4 gene. It is mapped to 1 C4, 1 39.55 cM. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.

Gene Full Name: Eph receptor A4

Gene ID: 13838

UniProt: Q03137

Pathways: RTK Signaling