PLAT CLIA Kit

Catalog No. ABIN8056098

This Chemiluminescent ELISA kit is designed for the quantitative measurement of Mouse PLAT.

Quick Overview for PLAT CLIA Kit (ABIN8056098)

Target: PLAT (Plasminogen Activator, Tissue (PLAT))

Reactivity: Mouse

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details PLAT CLIA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: CLIA Kit for Tissue Plasminogen Activator (tPA)

Analytical Method: Quantitative

Specificity:

This assay has high sensitivity and excellent specificity for detection of Tissue Plasminogen Activator (tPA).
No significant cross-reactivity or interference between Tissue Plasminogen Activator (tPA) and analogues was observed.

Sensitivity: < 0.056 ng/mL

Components:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• Substrate A
• Substrate B
• Wash Buffer (30X)
• Instruction manual

Material not included:

• Luminometer capable of reading 96-well microplates with the following parameters: lag time 30.0secs; read time 1.0 sec/well
• Single or multi-channel pipettes with high precision and disposable tips.
• Microcentrifuge Tubes.
• Deionized or distilled water.
• Absorbent paper for blotting the microplate.
• Container for Wash Solution.
• 0.01mol/L (or 1×) Phosphate Buffered Saline(PBS), pH7.0-7.2

Application Details

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Assay Precision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Plasminogen Activator (tPA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Plasminogen Activator (tPA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Storage: 4 °C,-20 °C

Storage Comment:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Expiry Date: 6 months

Target Details

Target: PLAT (Plasminogen Activator, Tissue (PLAT))

Alternative Name: Tissue Plasminogen Activator

Background: PLAT, T-PA, t-plasminogen activator, Alteplase, Reteplase

UniProt: P11214

Pathways: Autophagy, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, SARS-CoV-2 Protein Interactome