IL-2 ELISA Kit

Catalog No. ABIN8057091

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse IL-2.

Quick Overview for IL-2 ELISA Kit (ABIN8057091)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.05 pg/mL - 15 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Grade: High Sensitivity

Product Details IL-2 ELISA Kit

Minimum Detection Limit: 0.05 pg/mL

Analytical Method: Quantitative

Specificity: IL-2

Components:

• Plate
• Standard
• Diluent

Material not included: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-2.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: protect your eyes

Storage: 4 °C

Storage Comment: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2

Background: Interleukin-2 (IL-2) is a 15-16 kDa monomeric, α-helical glycoprotein that belongs to the Type I cytokine family. Mouse IL-2 is synthesized as a 169 amino acid (aa) precursor that contains a 20 aa signal sequence and a 149 aa mature chain, and mature mouse IL-2 shares 56 % and 73 % aa sequence identity with mature human and rat IL-2. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 has key roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells. IL-2 also promotes the differentiation of T cells into effector T cells and into memory T cells when the initial T cells is also stimulated by an antigen, thus helping the body fight off infections. Monitoring of IL-2 levels in serum provides more detailed insights in several pathological situations such as cancer, infectious diseases, transplant rejection, multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus and type I diabetes.

Gene ID: 16183

NCBI Accession: NM_008366

UniProt: P04351

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation